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Protistan parasite QPX of hard-shell clam Mercenaria mercenaria is a member of Labyrinthulomycota

2000; Inter-Research; Volume: 42; Linguagem: Inglês

10.3354/dao042185

1616-1580

Mark A. Ragan, G S MacCallum, Colleen Murphy, Jamie J. Cannone, Robin R. Gutell, S. E. McGladdery,

Aquatic Invertebrate Ecology and Behavior

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 42:185-190 (2000) - doi:10.3354/dao042185 Protistan parasite QPX of hard-shell clam Mercenaria mercenaria is a member of Labyrinthulomycota Mark A. Ragan1,2,*, Greg S. MacCallum3, Colleen A. Murphy1, Jamie J. Cannone4, Robin R. Gutell4, Sharon E. McGladdery3 1Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford St, Halifax, Nova Scotia B3H 3Z1, Canada 2Canadian Institute for Advanced Research, Program in Evolutionary Biology, 180 Dundas Street West, Suite 1400, Toronto, Ontario M5G 1Z8, Canada 3Fisheries and Oceans Canada, PO Box 5030, Moncton, New Brunswick E1C 9B6, Canada 4Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712-1095, USA *Present address: Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia. E-mail: m.ragan@imb.uq.edu.au ABSTRACT: Biomass of the protistan parasite QPX (quahaug parasite X) of hard-shell clam Mercenaria mercenaria was enriched from in vitro culture. The nuclear gene encoding the 18S RNA of the small-subunit ribosomal (ssu-rDNA) was recovered using the polymerase chain reaction (PCR) and sequenced. Phylogenetic analysis clearly showed that QPX is a member of phylum Labyrinthulomycota, within which it appears as a specific relative of Thraustochytrium pachydermum. These results confirm the provisional assignment of QPX to the Labyrinthulomycota made previously on the basis of morphological and ultrastructural characters found in some, but not all, geographic isolates. KEY WORDS: QPX, quahaug parasite X · Mercenaria mercenaria · Hard-shell clam · Small-subunit ribosomal DNA · Labyrinthulomycota · Thraustochytrium pachydermum Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 42, No. 3. Online publication date: September 28, 2000 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2000 Inter-Research.