Isolation, primary structure and synthesis of leucokinins VII and VIII: The final members of this new family of cephalomyotropic peptides isolated from head extracts of leucophaea maderae
1987; Elsevier BV; Volume: 88; Issue: 1 Linguagem: Inglês
10.1016/0742-8413(87)90043-0
ISSN1878-1969
AutoresG.Mark Holman, Benjamin J. Cook, R.J. Nachman,
Tópico(s)Chemical Synthesis and Analysis
Resumo1. Two additional octapeptides, belonging to the leucokinin family of cephalomyotropins, were isolated from head extracts of the cockroach, Leucophaea maderac. 2. A series of 4 high-performance liquid-chromatographic (HPLC) fractionations yielded sufficient quantities of pure peptides for micro amino acid analysis and primary sequence determination. The sequences obtained for the two peptides were: Asp-Pro-Ala-Phe-Ser-Ser-Trp-Gly-NH2 and Gly-Ala-Ser-Phe-Tyr-Ser-Trp-Gly-NH2. They were designated leucokinins VII (L-VII) and VIII (L-VIII) respectively. 3. The minimum concentrations of synthetic L-VII and L-VIII required to evoke a response from the isolated cockroach hindgut were 1.3 × 10−10 M and 2.8 × 10−11 M respectively. 4. L-VII and L-VIII are the lowest titre peptides of the leucokinin series (0.09 and 0.06 picomoles/head equivalent respectively). These are the final leucokinins isolated from our extracts.
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