Enhanced imaging and accelerated photothermalysis of A549 human lung cancer cells by gold nanospheres
2008; Future Medicine; Volume: 3; Issue: 5 Linguagem: Inglês
10.2217/17435889.3.5.617
ISSN1748-6963
AutoresXiong Liu, Mark C. Lloyd, Inna V. Fedorenko, Priya Bapat, Tatyana Zhukov, Qun Huo,
Tópico(s)Biosensors and Analytical Detection
ResumoBackground & aims: Gold nanoparticles are excellent photon-thermal energy converters. The purpose of this work was to investigate the influence of gold nanoparticles on the photothermalysis of A549 lung tumor cells. Materials & methods: A549 lung tumor cells were exposed to goat antihuman immunoglobulin (Ig)G-conjugated gold nanospheres (40 nm) and were then imaged under a dark-field microscope. The live cells were then subjected to photoirradiation using a 633-nm laser at different power levels. The viability of tumor cells under laser irradiation was monitored by confocal microscopy using a viability-assay kit. Results & discussion: The death rates of A549 lung tumor cells after gold nanoparticle exposure increased significantly under laser irradiation. The maximum initial cell death rate was observed at a laser power level of 3.75 mW, with the initial deactivation rate accelerated by a factor of 6.6 and a total loss of 92% of cell viability. Conclusion: This work demonstrated potential applications of gold nanospheres as both imaging probes and enhancing agents for photothermal therapy of cancer.
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