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Rhodovulum imhoffii sp. nov.

2007; Microbiology Society; Volume: 57; Issue: 2 Linguagem: Inglês

10.1099/ijs.0.64470-0

1466-5034

T. N. R. Srinivas, P. Anil Kumar, Ch. Sasikala, Ch. V. Ramana,

Identification and Quantification in Food

A yellowish-brown bacterium was isolated from photoheterotrophic enrichment cultures obtained from water samples of an aquaculture pond at Bhimunipatnam, India. Enrichment and isolation in a medium containing 2 % NaCl (w/v) yielded strain JA125 T , the cells of which were rod-shaped and non-motile. On the basis of the 16S rRNA gene sequence, strain JA125 T belongs to the class Alphaproteobacteria and is closely related to the type strains of Rhodovulum iodosum (96 %), Rhodovulum adriaticum (95 %), Rhodovulum robiginosum (95 %), Rhodovulum sulfidophilum (94 %) and Rhodovulum marinum (94 %). The cells of strain JA125 T contained vesicular internal membranes and bacteriochlorophyll a and carotenoids of the spheroidene series. Strain JA125 T grew optimally at 28 °C and at pH 7.0–8.0. The best growth occurred photoheterotrophically with a number of organic compounds serving as carbon sources and electron donors. The strain grew photoautotrophically, but chemoautotrophic growth did not occur. Strain JA125 T was able to utilize sulfide, sulfate, thiosulfate and thioglycolate as sulfur sources. Biotin was required as a growth factor. The DNA G+C content was 58 mol%. On the basis of 16S rRNA gene sequence analysis and the morphological and physiological data, strain JA125 T is significantly different from other species of the genus Rhodovulum and represents a novel species, for which the name Rhodovulum imhoffii sp. nov. is proposed. The type strain is JA125 T (=JCM 13589 T =DSM 18064 T ).