Portal de Periódicos da CAPES

The reaction of iodoacetate and iodoacetamide with proteins as determined with a silver/silver iodide electrode

1961; Elsevier BV; Volume: 48; Issue: 2 Linguagem: Inglês

10.1016/0006-3002(61)90488-7

1878-2434

David C. Watts, B. R. Rabin, E. M. Crook,

Adipose Tissue and Metabolism

An iodide electrode suitable for measuring the iodide produced in the reaction of iodocompounds with low concentrations of thiols is described. It has been used to determine the number of SH groups in proteins and other thiol compounds. The presence of two reactive thiol groups in creatine phosphokinase is confirmed and it is shown that their rate of alkylation parallels loss of enzyme activity. Urea-denatured ovalbumin is shown to react rapidly with iodoacetate to produce two equivalents of iodide per mole, after which a slow reaction, in which a further 9.8 equivalents are liberated, is observed. Native ovalbumin is shown to undergo a slow rection with iodoacetamide.