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Antimicrobial evaluation of copper sulfate (II) on strains of Enterococcus faecalis. In vitro study

2013; Universidad de Concepción.; Volume: 2; Issue: 3 Linguagem: Inglês

10.17126/joralres.2013.026

0719-2479

Marisol Sierra, Aldo Sanhueza, Raúl Alcántara, Gabriela Sánchez,

Occupational health in dentistry

Abstract: Introduction: Controlling Enterococcus faecalis is of vital importance in endodontics, as this pathogen is associated with endodontic failure. Experimental evidence has shown that copper has antibacterial activity against other pathogens with similar characteristics. The objective of this study was to determine the antimicrobial activity of copper (II) or cupric (SC-II) sulfate on strains of Enterococcus faecalis and to compare it with the most commonly used antimicrobials. Methodology: We used 33 strains of Enterococcus faecalis isolated from different clinical pictures in different Chilean hospitals. The minimum inhibitory concentration (MIC) of SC-II, chlorhexidine and calcium hydroxide was determined by the broth microdilution technique, following the recommendations given by the Clinical and Laboratory Standards Institute. Results: The MIC for CHX varied in the range of 5-10 µg/ml; SC-II from 1.5 to 12 µg/ml, and HC was >32 mg/ml. The geometric mean of SC-II was 6 µg/ml, lower than that of CHX, which was 7.29 µg/ml. Conclusions: SC-II showed antimicrobial activity at lower concentrations than CHX. HC (which could have been affected by the buffer effect of the broth microdilution technique) showed high values, not comparable to other compounds. We suggest carrying out further studies on the properties of SC-II, such assessing its biocompatibility and reaction with other materials to be used clinically in endodontic therapy. Keywords: “Enterococcus faecalis”[MeSH], “Copper sulfate”[MeSH], “Chlorhexidine”[MeSH], “Calcium Hydroxyde”[MeSH], “Endodontics”[MeSH]. Evaluacion antimicrobiana del sulfato de cobre (II) sobre cepas de Enterococcus faecalis. Estudio in vitro. Resumen: Introduccion: El control de Enterococcus faecalis es de vital importancia en endodoncia, ya que este patogeno esta asociado al fracaso endodontico. Evidencias experimentales que han demostrado que el cobre presenta actividad antibacteriana en otros patogenos de similares caracteristicas. El objetivo de este estudio es determinar la actividad antimicrobiana del sulfato de cobre (II) o cuprico (SC-II) sobre cepas de Enterococcus faecalis y compararla con los antimicrobianos mas usados en la actualidad. Metodologia: Estudio in vitro. Se utilizo la tecnica de microdilucion en caldo segun lineamientos del Clinical and Laboratory Standards Institute, incluyendo 33 cepas de Enterococcus faecalis obtenidas desde hospitales chilenos, para cada una de las cuales se determino las concentraciones minimas inhibitorias (CMI) de: SC-II, Clorhexidina (CHX) e Hidroxido de calcio (HC). Resultados: La CMI para CHX tuvo un rango de 5-10 µg/ml, el SC-II de 1,5-12 mM y el HC fue >32 mg/ml. Estas diferencias fueron estadisticamente significativas entre los 3 antimicrobianos utilizados (p<0,001). Conclusiones: El SC-II mostro actividad antimicrobiana a bajas concentraciones, superiores a CHX, pero menores a HC (que pudo ser afectado por el efecto tampon de la tecnica de microdilucion en caldo). Se sugiere seguir con los estudios de las propiedades del SC-II, como evaluacion de biocompatibilidad y reaccion con otros materiales para ser utilizados clinicamente en la terapia endodontica. Palabras clave: Enterococcus faecalis, sulfato de cobre, clorhexidina, hidroxido de calcio, endodoncia.