Identification of Autonomously Replicating Sequence (ARS) Elements in Eukaryotic Cells
1997; Elsevier BV; Volume: 13; Issue: 3 Linguagem: Inglês
10.1006/meth.1997.0522
ISSN1095-9130
AutoresRosemary K. Clyne, Thomas J. Kelly,
Tópico(s)Genomics and Chromatin Dynamics
ResumoAutonomously replicating sequence (ARS) elements were first identified in the budding yeastSaccharomyces cerevisiaeas chromosomal DNA fragments that promoted high frequency of transformation and extrachromosomal maintenance of plasmid DNA. These specific sequence elements were subsequently shown to function as origins of DNA replication. Detailed analysis of the structure and function of ARS elements has been limited largely toS. cerevisiaeand more recently the fission yeastSchizosaccharomyces pombe.Characterization of ARS activity in other eukaryotes is far less complete. Here we describe the ARS assay developed in yeast and its application to the study of origin function in other eukaryotes. Other available methods for detecting autonomous replication in these systems are also presented.
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