Somaclonal Variation of Scopolamine Content in Protoplast-Derived Cell Culture Clones of Hyoscyamus muticus
1986; Thieme Medical Publishers (Germany); Volume: 52; Issue: 01 Linguagem: Inglês
10.1055/s-2007-969053
ISSN1439-0221
AutoresKirsi‐Marja Oksman‐Caldentey, André Strauss,
Tópico(s)Potato Plant Research
ResumoWe have established clonal cell cultures derived from protoplasts of haploid and diploid Hyoscyamus muticus L. var. Gatersleben (Solanaceae) plants. The scopolamine content of plants and the clones was determined by sensitive enzyme- and radioimmunoassay. Variation in scopolamine content was significant among whole plants. Scopolamine content in haploid plants was found to be clearly 2-3 times higher than in diploid ones. The same difference was observed between the cell culture clones derived from haploid plants compared to the clones derived from diploids. The mean content of scopolamine in cultured cell clones was much smaller than in plants, usually by about a factor of 200. There was also substantial somaclonal variation in scopolamine content of various clones derived from individual haploid and diploid plants. Possible sources and reasons for this variation were tested.
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