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Disulfide-Linked, Gold Nanoparticle Based Reagent for Detecting Small Molecular Weight Thiols

2009; American Chemical Society; Volume: 131; Issue: 7 Linguagem: Inglês

10.1021/ja808548x

1943-2984

Suzanne Durocher, Asad Rezaee, Caroline Hamm, Chitra Rangan, Silvia Mittler, Bülent Mutus,

Nanocluster Synthesis and Applications

Disulfide-linked gold nanoparticles (AuNP) were synthesized by reacting dithiobis[succinimidylpropionate] (DSP) coated nanoparticles with glutathione disulfide. AuNP-cross-linking was monitored by the red shift and broadening of the AuNP's localized surface plasmon absorption resonance (LSPR) spectrum. The exposure of the disulfide-linked AuNPs to a variety of free thiols with systematically varying molecular weight revealed a AuNP-disulfide stability to reduction by thiols up to a critical molecular weight, Mc, of >300 Da thus making the disulfide-linked AuNP the first reagent that can discriminate thiols based on their size.