Protein kinase C-α and -β play antagonistic roles in the differentiation process of THP-1 cells
2000; Elsevier BV; Volume: 12; Issue: 5 Linguagem: Inglês
10.1016/s0898-6568(00)00069-3
ISSN1873-3913
Autores Tópico(s)Melanoma and MAPK Pathways
ResumoThe roles of protein kinase C (PKC) isoenzymes in the differentiation process of THP-1 cells are investigated. Inhibition of PKC by RO 31-8220 reduces the phagocytosis of latex particles and the release of superoxide, prostaglandin E2 (PGE2), and tumour necrosis factor (TNF)-α. The proliferation of THP-1 cells is slightly enhanced by RO 31-8220. Stable transfection of THP-1 cells with asPKC-α, and incubation of THP-1 cells with antisense (as) PKC-α oligodeoxynucleotides reduces PKC-α levels and PKC activity. asPKC-α-transfected THP-1 cells show a decreased phagocytosis and a decreased release of superoxide, PGE2 and TNF-α. The proliferation of asPKC-α-transfected THP-1 cells is enhanced. Stable transfection of THP-1 cells with asPKC-β, and incubation of THP-1 cells with asPKC-β oligodeoxynucleotides, reduces PKC-β levels and PKC activity. asPKC-β-transfected THP-1 cells show a decreased phagocytosis, a decreased TNF-α release, and a decreased proliferation. However, no difference is measured in the release of superoxide and PGE2. These results suggest that: (1) PKC-α but not PKC-β is involved in the release of superoxide and PGE2; (2) TNF-α release and the phagocytosis of latex particles are mediated by PKC-α, PKC-β, and other PKC isoenzymes; and (3) PKC-α and PKC-β play antagonistic roles in the differentiation process of THP-1 cells. PKC-α promotes the differentiation process of THP-1 cells, PKC-β retards the differentiation of THP-1 cells into macrophage-like cells.
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