Detection of Human Alpha-herpesvirus DNA using Consensus Primers and Specific Probes
1994; Taylor & Francis; Volume: 114; Issue: sup514 Linguagem: Inglês
10.3109/00016489409127577
ISSN1651-2251
AutoresToshiya Aono, Shingo Murakami, Naoaki Yanagihara, Koichi Yamanishi,
Tópico(s)Cytomegalovirus and herpesvirus research
ResumoA simple and rapid diagnostic assay system was developed for detecting and identifying human alpha-herpesviruses, such as herpes simplex virus types 1 and 2 and varicella-zoster virus, by polymerase chain reaction. This system was based on an amplification step, using primers that bind the DNA polymerase consensus sequence of alpha-human herpesviruses, and a detection step, using non-radioactive virus-specific probes. This method could be used to amplify any human alpha-herpesviruses, and each virus-specific probe was highly specific for identification of the amplified product. This system is readily applicable for implementation in the clinical laboratory.
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