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Physical mapping of the Mycoplasma capricolum genome

1991; Oxford University Press; Volume: 79; Issue: 2-3 Linguagem: Inglês

10.1111/j.1574-6968.1991.tb04550.x

1574-6968

Makoto Miyata, Lu Wang, T Fukumura,

Aquaculture disease management and microbiota

A physical map of Mycoplasma capricolum ATCC 27343 genome was constructed, based on estimation of the restriction fragment sizes by pulse-field electrophoresis. The linkage order of restriction fragments was determined by two-dimensional electrophoresis of partial and complete single digests and complete double digests and by Southern hybridization analysis. The genome size was established at 1155.5 kb, and 26 cleavage sites for 7 endonucleases were assigned to the map.