Influence of medium composition on the accumulation of flavonolignans in cultured cells of Silybum marianum (L.) Gaertn
1999; Elsevier BV; Volume: 144; Issue: 2 Linguagem: Inglês
10.1016/s0168-9452(99)00056-4
ISSN1873-2259
AutoresMargarita Cacho, Margarita Morán, Purificación Corchete, Jorge Fernández‐Tárrago,
Tópico(s)Thallium and Germanium Studies
ResumoFlavonolignan production was examined in callus and cell suspension cultures derived from cotyledons of Silybum marianum (L.) Gaertn. The flavonolignan content in 2-month-old calluses was lower than the values obtained for fruits (1.5%) and progressively decreased over repeated subcultures. Higher accumulation was observed in cell suspension cultures than in callus but continued to be very low with respect to the values obtained for fruits (14%). The growth and flavonolignan production of suspensions were tested using different concentrations of KNO3, KH2PO4, iron and calcium. Only the removal of calcium ions promoted flavonolignan accumulation, although under this condition, growth was significantly reduced.
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