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Genetic Transformation of Passionflower and Evaluation of R 1 and R 2 Generations for Resistance to Cowpea aphid borne mosaic virus

2011; American Phytopathological Society; Volume: 95; Issue: 8 Linguagem: Inglês

10.1094/pdis-12-10-0873

1943-7692

Alessandra Cristina B. A. Monteiro-Hara, Adriana Salomão Jadão, B. M. J. Mendes, Jorge Alberto Marques Rezende, Flavio Trevisan, A. P. O. A. Mello, Maria Lúcia Carneiro Vieira, Laura Maria Molina Meletti, Sônia Maria de Stéfano Piedade,

Plant tissue culture and regeneration

We report on the production and evaluation of passionflower transgenic lines for resistance to Cowpea aphid borne mosaic virus (CABMV). Genetic transformation was done using Agrobacterium tumefaciens and transgene integration was confirmed by Southern blot analyses, resulting in nine transgenic lines for ‘IAC 275’ and three for ‘IAC 277’. Transgenic lines were clonally propagated and evaluated for resistance to CABMV. After the third inoculation, under higher inoculum pressure, only propagated plants of the transgenic line T16 remained asymptomatic, indicating a high resistance to infection with CABMV. This transgenic line was self-pollinated and the R 1 generation was evaluated together with the R 1 generation of another resistant transgenic line (T2) identified previously. Plants were inoculated with CABMV by means of viruliferous Myzus nicotianae. All 524 T2R 1 plants became infected, whereas 13 of 279 T16R 1 remained asymptomatic after four successive inoculations. A T16R 2 generation was obtained and plants were inoculated with CABMV mechanically or by aphids. After successive inoculations, 118 of 258 plants were symptomless, suggesting that the resistance to CABMV was maintained in the plant genome as the homozygous condition was achieved. Five selected resistant T16R 2 plants which contained the capsid protein gene are being crossed for further analyses.