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Detection of New Delhi Metallo-beta-Lactamase and Extended-Spectrum beta-Lactamase Genes in Escherichia coli Isolated from Mastitic Milk Samples

2013; Wiley; Volume: 60; Issue: 5 Linguagem: Inglês

10.1111/tbed.12119

1865-1682

Sandeep Ghatak, Anil Kumar Singha, Arnab Sen, C. Guha, Anuj Ahuja, Uttaran Bhattacharjee, Samir Das, N. R. Pradhan, K. Puro, C. Jana, Tushar Kumar Dey, K. L. Prashantkumar, Arun K. Das, I. Shakuntala, U. Biswas, Partha Sarathi Jana,

Probiotics and Fermented Foods

Transboundary and Emerging DiseasesVolume 60, Issue 5 p. 385-389 Rapid Communication Detection of New Delhi Metallo-beta-Lactamase and Extended-Spectrum beta-Lactamase Genes in Escherichia coli Isolated from Mastitic Milk Samples S. Ghatak, Corresponding Author S. Ghatak Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, India Correspondence: S. Ghatak. Division of Animal Health, ICAR Research Complex for NEH Region, Umiam 793103, Meghalaya, India. Tel./Fax: +91 364 2570071; E-mail: [email protected]Search for more papers by this authorA. Singha, A. Singha Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorA. Sen, A. Sen Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorC. Guha, C. Guha Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorA. Ahuja, A. Ahuja Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorU. Bhattacharjee, U. Bhattacharjee Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorS. Das, S. Das Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorN. R. Pradhan, N. R. Pradhan Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorK. Puro, K. Puro Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorC. Jana, C. Jana KVK-Central Research Institute for Jute and Allied Fibres, Budbud, IndiaSearch for more papers by this authorT. K. Dey, T. K. Dey Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorK. L. Prashantkumar, K. L. Prashantkumar Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorA. Das, A. Das Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorI. Shakuntala, I. Shakuntala Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorU. Biswas, U. Biswas Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorP. S. Jana, P. S. Jana Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this author S. Ghatak, Corresponding Author S. Ghatak Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, India Correspondence: S. Ghatak. Division of Animal Health, ICAR Research Complex for NEH Region, Umiam 793103, Meghalaya, India. Tel./Fax: +91 364 2570071; E-mail: [email protected]Search for more papers by this authorA. Singha, A. Singha Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorA. Sen, A. Sen Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorC. Guha, C. Guha Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorA. Ahuja, A. Ahuja Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorU. Bhattacharjee, U. Bhattacharjee Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorS. Das, S. Das Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorN. R. Pradhan, N. R. Pradhan Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorK. Puro, K. Puro Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorC. Jana, C. Jana KVK-Central Research Institute for Jute and Allied Fibres, Budbud, IndiaSearch for more papers by this authorT. K. Dey, T. K. Dey Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorK. L. Prashantkumar, K. L. Prashantkumar Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorA. Das, A. Das Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorI. Shakuntala, I. Shakuntala Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, IndiaSearch for more papers by this authorU. Biswas, U. Biswas Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this authorP. S. Jana, P. S. Jana Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, IndiaSearch for more papers by this author First published: 19 July 2013 https://doi.org/10.1111/tbed.12119Citations: 43Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary In this study, eight Escherichia coli isolates were obtained from milk samples of dairy cattle suffering from clinical/subclinical mastitis. Isolates were characterized for antimicrobial resistance traits and virulence genes. Results revealed that one isolate was harbouring New Delhi metallo-beta-lactamase gene (blaNDM). Cloning and sequencing of the PCR amplicon confirmed the identity of the gene (GenBank accession no. KC769583) having 100% homology with blaNDM-5 (GenBank accession no. JN104597.1), and this isolate was susceptible to colistin, chloramphenicol and tetracycline only. Moreover, another isolate carried extended-spectrum beta-lactamase (ESBL) gene – blaCTX-M, and all isolates possessed blaTEM gene. Of the eight isolates, only one isolate was positive for shiga toxin gene (stx2), and none were harbouring stx1 gene. Occurrence of New Delhi metallo-beta-lactamase (blaNDM) in one E. coli isolate and ESBL genes in other isolates poses a potential threat to human health following possible entry and spread through food chain. References Anonymous 2012: NDM-1 Carrying Enterobacteriaceae – USA (02): Feline. 20120916.1294263. Available at http://www.promedmail.org (accessed February 2, 2013). 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