A reliable Feulgen-acriflavine-SO2 staining procedure for quantitative DNA measurements.
1980; SAGE Publishing; Volume: 28; Issue: 9 Linguagem: Inglês
10.1177/28.9.6157711
ISSN1551-5044
Autores Tópico(s)Molecular Biology Techniques and Applications
ResumoFeulgen-acriflavine-SO2 staining was performed on chicken erythrocytes and rat liver cells under different cytochemical conditions. The obtained results were compared to conventional Feulgen-pararosaniline-SO2 staining by means of scanning cytophotometry and microfluorometry. It was found that if acid ethanol rinsing was included in the Feulgen-acriflavine-SO2 procedure, both Feulgen procedures were fully comparable in specificity and quantitative aspects.
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