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Effect of hyperthermia on the replication of white spot syndrome virus (WSSV) in Procambarus clarkii

2006; Inter-Research; Volume: 71; Linguagem: Inglês

10.3354/dao071175

1616-1580

Huahua Du, WF Li, ZR Xu, ZS Kil,

Insect symbiosis and bacterial influences

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 71:175-178 (2006) - doi:10.3354/dao071175 Effect of hyperthermia on the replication of white spot syndrome virus (WSSV) in Procambarus clarkii Hua-Hua Du, Wei-Fen Li, Zi-Rong Xu*, Zong-Su Kil Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou 310029, PR China *Corresponding author. Email: zrxu@zju.edu.cn ABSTRACT: The effect of hyperthermia on the development of white spot syndrome virus (WSSV) in the crayfish Procambarus clarkii was studied by competitive PCR. Crayfish were exposed to different temperatures (24 ± 1 and 32 ± 1°C) after WSSV injection. No mortality was observed when crayfish were held at 32 ± 1°C, but mortality reached 100% when crayfish were transferred to 24 ± 1°C. Competitive PCR showed that viral levels at 32 ± 1°C remained at 105 copies mg–1 tissue, while at 24 ± 1°C levels were significantly higher, rising from 104 to 1010 copies mg–1 tissue. These results suggest that hyperthermia reduces viral replication, but does not eliminate viral particles from WSSV-infected crayfish. KEY WORDS: White spot syndrome virus · Hyperthermia · Viral replication · Procambarus clarkii · Competitive PCR · Water temperature Full text in pdf format PreviousExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 71, No. 2. Online publication date: July 25, 2006 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2006 Inter-Research.