The role of complex formation in the neurotoxicity of crotoxin components A and B
1976; Elsevier BV; Volume: 14; Issue: 4 Linguagem: Inglês
10.1016/0041-0101(76)90024-6
ISSN1879-3150
AutoresR.A. Hendon, H. Fraenkel‐Conrat,
Tópico(s)Radiopharmaceutical Chemistry and Applications
ResumoCrotoxin can be reconstituted from its two protein components in a physical form resembling the native neurotoxin. Through use of radioactive labels, crotoxin can be shown to form the complex even in quite dilute solution (0.03 mg per ml), although both native and reconstituted crotoxin are under such conditions susceptible to dissociation by electrophoresis. Various indications were obtained that the biological synergism of components A and B is not dependent on physical complex formation. It actually appears probable that the complex would be dissociated at the extreme dilution occurring within the test animal. Studies of the killing rate of crotoxin, as compared to α and β bungarotoxin, indicated an intermediate behavior.
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