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Oxidase activity and lipid composition of respiratory particles from Claviceps purpurea (Ergot fungus)

1964; Elsevier BV; Volume: 107; Issue: 1 Linguagem: Inglês

10.1016/0003-9861(64)90266-8

1096-0384

Jane Anderson, Fengjiang Sun, J. Ken McDonald, V. H. Cheldelin,

Fungal Biology and Applications

Respiratory particles with complete succinate and DPNH oxidase systems were prepared from vegetative cultures of Claviceps purpurea. The rates were 4–10 μmoles of succinate per hour per milligram protein at 36 °C and 20–24 μmoles of DPNH oxidized per hour per milligram at 27 °. Beef heart cytochrome c increased oxidase activity and antimycin A and cyanide completely inhibited the oxidase activity. The succinate oxidase system is rapidly disintegrated by sonic irradiation. Claviceps purpurea respiratory particles contained 26% lipid and 58% protein. There were no plasmalogens. Claviceps purpurea respiratory particles contained 10 μmoles of phosphorus per milligram lipid. The percentage of the various phospholipids was phosphatidylinositol, 28; phosphatidylcholine, 27; phosphatidylserine, 13; phosphatidylethanolamine, 18; polyglycerophosphate, 7; and residue, 7. Coenzyme Q10 (H-10) is a component of the C. purpurea respiratory particles. Claviceps purpurea respiratory lipid was slightly more effective than beef heart mitochondrial lipid in restoring the succinate-cytochrome c reductase activity of acetone-extracted beef heart mitochondria.