Putative immunolocalization of the mechanoelectrical transduction channels in mammalian cochlear hair cells
1992; Royal Society; Volume: 248; Issue: 1323 Linguagem: Inglês
10.1098/rspb.1992.0064
ISSN1471-2954
AutoresCarole M. Hackney, David N. Furness, Dale Benos, John F. Woodley, J Barratt,
Tópico(s)Vestibular and auditory disorders
ResumoRestricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Hackney Carole M. , Furness David N. , Benos Dale J. , Woodley John F. and Barratt Joan 1992Putative immunolocalization of the mechanoelectrical transduction channels in mammalian cochlear hair cellsProc. R. Soc. Lond. B.248215–221http://doi.org/10.1098/rspb.1992.0064SectionRestricted accessArticlePutative immunolocalization of the mechanoelectrical transduction channels in mammalian cochlear hair cells Carole M. Hackney Google Scholar Find this author on PubMed Search for more papers by this author , David N. Furness Google Scholar Find this author on PubMed Search for more papers by this author , Dale J. Benos Google Scholar Find this author on PubMed Search for more papers by this author , John F. Woodley Google Scholar Find this author on PubMed Search for more papers by this author and Joan Barratt Google Scholar Find this author on PubMed Search for more papers by this author Carole M. Hackney Google Scholar Find this author on PubMed , David N. Furness Google Scholar Find this author on PubMed , Dale J. Benos Google Scholar Find this author on PubMed , John F. Woodley Google Scholar Find this author on PubMed and Joan Barratt Google Scholar Find this author on PubMed Published:22 June 1992https://doi.org/10.1098/rspb.1992.0064AbstractHair cells bear an apical bundle of stereocilia arranged in serried rows. Deflection of the bundle controls the opening and closing of mechanoelectrical transduction channels, thereby altering the conductance across the apical plasma membrane. Two locations for these channels have been proposed in the bundle, either near the bases of the stereocilia or towards their tips. One hypothesis that is consistent with the latter possibility suggests that fine extracellular filaments, which run between the tips of the shorter stereocilia and the sides of the taller stereocilia behind, operate the channels. Determining the precise position of the channels is essential to test this hypothesis. We have therefore attempted to localize them immunocytochemically. Because hair-cell transduction is amiloride sensitive, the channels may have an amiloride-binding site associated with them. We have therefore used a polyclonal antibody raised against another amiloride-sensitive ion channel to hunt for them. This antibody recognizes a 62-64 kDa band in immunoblots of cochlear tissue, and produces discrete labelling in the hair bundle. This is most concentrated just below the tips of the shorter stereocilia, coinciding with a region of specialization in the closely apposed membranes of the short and tall stereocilia but not with either end of the tip link.FootnotesThis text was harvested from a scanned image of the original document using optical character recognition (OCR) software. As such, it may contain errors. Please contact the Royal Society if you find an error you would like to see corrected. Mathematical notations produced through Infty OCR. Previous ArticleNext Article VIEW FULL TEXT DOWNLOAD PDF FiguresRelatedReferencesDetailsCited by Ohmori H (2019) Hair Cell Mechano-electrical Transduction and Synapse Transmission Auditory Information Processing, 10.1007/978-981-32-9713-5_1, (1-41), . 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Warren B and Nowotny M (2021) Bridging the Gap Between Mammal and Insect Ears – A Comparative and Evolutionary View of Sound-Reception, Frontiers in Ecology and Evolution, 10.3389/fevo.2021.667218, 9 Hackney C and Furness D (2013) The composition and role of cross links in mechanoelectrical transduction in vertebrate sensory hair cells, Journal of Cell Science, 10.1242/jcs.106120 Alagramam K, Goodyear R, Geng R, Furness D, van Aken A, Marcotti W, Kros C, Richardson G and Coleman M (2011) Mutations in Protocadherin 15 and Cadherin 23 Affect Tip Links and Mechanotransduction in Mammalian Sensory Hair Cells, PLoS ONE, 10.1371/journal.pone.0019183, 6:4, (e19183) This Issue22 June 1992Volume 248Issue 1323 Article InformationDOI:https://doi.org/10.1098/rspb.1992.0064PubMed:1354359Published by:Royal SocietyPrint ISSN:0962-8452Online ISSN:1471-2954History: Manuscript received20/01/1992Manuscript accepted02/03/1992Published online01/01/1997Published in print22/06/1992 License:Scanned images copyright © 2017, Royal Society Citations and impact Large datasets are available through Proceedings B's partnership with Dryad
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