Host modulation of tissue destruction caused by periodontopathogens: effects on a mixed microbial infection composed ofPorphyromonas gingivalisandFusobacterium nucleatum
1997; Elsevier BV; Volume: 23; Issue: 1 Linguagem: Inglês
10.1006/mpat.1996.0129
ISSN1096-1208
AutoresJeffrey L. Ebersole, Frank Feuille, Lakshmyya Kesavalu, Stanley C. Holt,
Tópico(s)HIV/AIDS oral health manifestations
ResumoThese studies determined the ability of selected periodontopathogens to synergistically initiate soft tissue destruction in a murine abscess model. The development of immunity following recovery from infection or by active immunization was also examined. Mice were infected withP. gingivalisW50,F. nucleatumT18, or a combination of the two microorganisms.F. nucleatumcaused only a localized lesion in contrast toP. gingivaliswhich caused a spreading suppurative inflammatory lesion of the skin and subcutaneous tissues, which, depending upon the dose, could result in death. Infection of mice with a combination ofP. gingivalisandF. nucleatumelicited a significantly greater lesion size (P<0.001) and lethality compared withP. gingivalisalone. Mice infected with a subclinical dose (no visible lesion) ofP. gingivalisfailed to develop protective immunity to a secondaryP. gingivalischallenge. Mice that had recovered fromP. gingivalislesions demonstrated partial protection against subsequentP. gingivalischallenge; however, the immunity was less protective against the mixedF. nucleatum+P. gingivalisinfection. Active immunization withP. gingivalisprotected against both theP. gingivalisandF. nucleatum+P. gingivalischallenges and this protection was correlated with the levels of specific serum immunoglobulin G (IgG) antibody. The results indicated that the murine model is ideally suited to examine bacterially-mediated mixed infections that result in soft tissue destruction. This destruction can be minimized, but not abrogated, with development of immunity. Challenge with sufficient numbers of the pathogens can overwhelm the acquired immunity.
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