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Quantitative Analysis by Liquid Chromatography–Tandem Mass Spectrometry of Deuterium-Labeled and Unlabeled Vitamin E in Biological Samples

2001; Elsevier BV; Volume: 289; Issue: 1 Linguagem: Inglês

10.1006/abio.2000.4913

1096-0309

Charlotte Lauridsen, Scott W. Leonard, Donald A. Griffin, D.C. Liebler, Thomas D. McClure, Maret G. Traber,

Coenzyme Q10 studies and effects

A method for quantification of unlabeled α-tocopherol and the deuterated tocopherols, RRR-α-5-(CD3)-tocopherol (d3RRR) and all rac-α-5,7-(CD3)2 tocopherol (d6all-rac) in plasma by HPLC–tandem mass spectrometry (LC-MS/MS) has been developed. Deuterated and unlabeled α-tocopherols were separated by HPLC and were detected by positive ion multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with a heated nebulizer–atmospheric pressure chemical ionization interface, following routine extraction of vitamin E from plasma. The accuracy and precision were evaluated by replicate analysis of standards and samples. Human plasma samples, which were obtained at different times after the subject had consumed a capsule containing 1:1 ratio of d3RRR and d6all rac-α-tocopheryl acetates, were analyzed with this method. Plasma deuterated α-tocopherols measured by LC-MS/MS followed the same pattern as previously demonstrated by GC-MS measurement, without requiring an extra derivitization step. The detection limit was 10 pmol for each form of α-tocopherol injected.