The indirect UV detection in the analysis of ursodeoxycholic acid and related compounds by HPCE
1997; Elsevier BV; Volume: 16; Issue: 2 Linguagem: Inglês
10.1016/s0731-7085(97)00034-4
ISSN1873-264X
AutoresM.G. Quaglia, A. Farina, E. Bossù, Caterina Dell’Aquila, A. Doldo,
Tópico(s)Analytical Chemistry and Chromatography
ResumoA high-performance capillary zone electrophoretic (HPCE) assay has been developed for the determination of ursodeoxycholic acid (UDCA) and its usual impurities. Considering the low molecular absorptivity of UDCA and its related compounds indirect UV detection was used. The electrophoretic capillary was filled with a background electrolyte (BGE) containing an UV absorbing ion: benzoic acid (BA) or 5,5-diethylbarbituric acid (DBA). To enhance the selectivity of the assay diimethyl-beta-cyclodextrines (D-beta-CDs) or trimethyl-beta-cyclodextrines (T-beta-CDs) have been added to the running buffer together with methylcellulose or urea. All considered impurities were well resolved with two buffers studied, with the exception of methylursodehoxycholate, a neutral compound.
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