Abortive infection of Escherichia coli with the bacteriophage f1: DNA synthesis associated with the membrane
1973; Elsevier BV; Volume: 55; Issue: 1 Linguagem: Inglês
10.1016/s0042-6822(73)81006-2
ISSN1096-0341
AutoresArnold S. Grandis, Robert E. Webster,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoDNA synthesis associated with the membrane of Escherichia coli infected with f1 amber mutant bacteriophage was studied. [Methyl-3H] thymidine was used to radioactively label the DNA being synthesized during the period that macromolecular synthesis rapidly declines in these abortively infected bacteria. DNA-membrane complexes were isolated and washed with a low salt buffer to remove loosely associated DNA. The remaining DNA was released with Sarkosyl and analyzed by neutral, high salt, sucrose gradients to determine the nature of this membrane-associated DNA. The synthesized DNA in tight association with the membrane was predominantly phage DNA in the form of f1 RFI DNA molecules. The two strands of the f1 RFI DNA were symmetrically labeled, as if phage DNA synthesis were unable to switch effectively to f1 single stranded DNA formation. These events lead to the accumulation of progeny f1 RFI DNA on the membrane of these abortively infected bacteria.
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