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Human colostrum: Identification of minor proteins in the aqueous phase by proteomics

2006; Wiley; Volume: 6; Issue: 7 Linguagem: Inglês

10.1002/pmic.200500558

1615-9861

David J. Palmer, Van Kelly, Anne‐Marie Smit, Sulee Kuy, Catriona Knight, Garth J. S. Cooper,

Animal health and immunology

Abstract Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha‐lactalbumin and HSA by immunoabsorption, using 2‐D LC and gel‐based proteomic methods. One hundred and fifty‐one proteins were identified, 83 of which have not been previously reported in human colostrum, or milk. This is the first comprehensive proteomic analysis of human colostrum produced during the first 48 h of lactation.