Anomalous Results Obtained in the Determination of Available Lysine in Casein Using 2,4,6-Trinitrobenzenesulfonic Acid
1970; Elsevier BV; Volume: 53; Issue: 11 Linguagem: Inglês
10.3168/jds.s0022-0302(70)86450-5
ISSN1529-9066
AutoresV.H. Holsinger, Linda P. Posati, M.J. Pallansch,
Tópico(s)Probiotics and Fermented Foods
ResumoWhen acid precipitated caseins were evaluated for their available lysine using 2,4,6-trinitrobenzenesulfonie acid, amounts greater than the total lysine content were found.Detailed investigation of several casein fractions showed that the a q variants and fl casein yielded available lvsine amounts consistent with total lysine.However, available ]ysine of the K-fraction was higher than its total lysine content.This is ascribed to hexosamines which are part of the structure of this molecular species.This finding indicates that caution should be observed in using 2,4,6-trinitrobenzenesulfonic acid for the determination of available lysine in foods containing significant amounts of glycoproteins.
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