Pre‐symptomatic detection of prions by cyclic amplification of protein misfolding
2004; Wiley; Volume: 579; Issue: 3 Linguagem: Inglês
10.1016/j.febslet.2004.12.035
ISSN1873-3468
AutoresClaudio Soto, Laurence Anderes, Silvia Suardi, Franco Cardone, Joaquı́n Castilla, Marie-Jose Frossard, Sergio Peano, Paula Saá, Lucia Limido, Michaela Carbonatto, James W. Ironside, Juan María Torres, Maurizio Pocchiari, Fabrizio Tagliavini,
Tópico(s)Trace Elements in Health
ResumoTransmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders affecting humans and animals. At present, it is not possible to recognize individuals incubating the disease before the clinical symptoms appear. We investigated the effectiveness of the “Protein Misfolding Cyclic Amplification” (PMCA) technology to detect the protease‐resistance disease‐associated prion protein (PrP res ) in pre‐symptomatic stages. PMCA allowed detection of PrP res in the brain of pre‐symptomatic hamsters, enabling a clear identification of infected animals as early as two weeks after inoculation. Furthermore, PMCA was able to amplify minute quantities of PrP res from a variety of experimental and natural TSEs. Finally, PMCA allowed the demonstration of PrP res in an experimentally infected cow 32 month post‐inoculation, that did not show clinical signs and was negative by standard Western blot analysis. Our findings indicate that PMCA may be useful for the development of an ultra‐sensitive diagnostic test to minimize the risk of further propagation of TSEs.
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