Sensitive fluorescence detection of some nitrosamines by precolumn derivatization with dansyl chloride and high-performance liquid chromatography
1992; Elsevier BV; Volume: 589; Issue: 1-2 Linguagem: Inglês
10.1016/0021-9673(92)80044-u
ISSN1873-3778
AutoresZhi Wang, Hongda Xu, Chengguang Fu,
Tópico(s)Polyamine Metabolism and Applications
ResumoA fluorescence detection method for the determination of some nitrosamines by precolumn derivatization with dansyl chloride and high-performance liquid chromatography was developed. The separation, derivatization and detection conditions were optimized. The sample is first denitrosated by hydrobomic acid-acetic acid to produce secondary amines, which are then subjected to reaction with dansyl chloride to form dansyl derivatives. The reaction mixtures are injected and separated on a C18 column with acetonitrile-water (73:27, v/v) as mobile phase with fluorimetric detection at 530 nm (excitation at 350 nm). The method shows high sensitivity and precision. The detection limits are 0.06 ng for N-nitrosodimethylamine and N-nitrosodiethylamine, 0.09 ng for N-nitrosodipropylamine and 0.16 ng for N-nitrosodibutylamine, with relative standard deviations of 2.1, 1.9, 2.1 and 1.5%, respectively. The linearity of the calibration graphs covers more than two orders of magnitude of concentration of the compounds, and the correlation coefficients are 0.9995.
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