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Protoplasts from Penicillium glaucum

1960; Nature Portfolio; Volume: 188; Issue: 4748 Linguagem: Inglês

10.1038/188426a0

1476-4687

G Meinecke,

Fungal Biology and Applications

Bachmann and Bonner1 were able to obtain free protoplasts from Neurospora crassa, if this fungus was cultivated in thick liquid media, such as 20 per cent sucrose and 10 per cent enzyme preparations. Protoplasts emerge through pores in the hyphal walls. The authors showed, by means of a microscopical photo-series, that the protoplasts regenerate to form a complete new fungus mycelium.