Partial purification of a toxin from tentacles of the sea anemone Anemonia sulcata
1973; Elsevier BV; Volume: 11; Issue: 5 Linguagem: Inglês
10.1016/0041-0101(73)90116-5
ISSN1879-3150
AutoresValentina Novak, Dušan Sket, Gregor Cankar, D. Lebez,
Tópico(s)Venomous Animal Envenomation and Studies
ResumoA toxin of low molecular weight was isolated from tentacles of the sea anemone Anemonia sulcata P. Partially purified toxin was obtained by precipitation with ammonium sulphate, ion exchange chromatography on DEAE cellulose and chromatography on Sephadex G-50. The molecular weight of a partially purified toxin, probably a basic protein was approximately 6000 as determined by gel filtration. Disc electrophoresis of the partially purified toxin revealed four proteins. Heating for 15 min at 90°C inactivated the toxin. The toxic activity remained unchanged over 12 months when stored in frozen solution at −26°C. The ld100 is 6 mg of the toxin per kg (rat) by i.v. injection. The toxin showed a cardiotropic action on the rat heart. When injected i.v. the toxin caused cardiac arrhythmia and fall of the arterial blood pressure.
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