High‐yield expression of a viral peptide vaccine in transgenic plants
2001; Wiley; Volume: 488; Issue: 1-2 Linguagem: Inglês
10.1016/s0014-5793(00)02405-4
ISSN1873-3468
AutoresFélix Gil-Dones, Alejandro Brun, Andrés Wigdorovitz, Rafael Catalá, Jorge L. Martı́nez-Torrecuadrada, J. Ignacio Casal, Julio Salinas, Manuel V. Borca, José M. Escribano,
Tópico(s)Plant Virus Research Studies
ResumoA high‐yield production of a peptide vaccine in transgenic plants is described here. A 21‐mer peptide, which confers protection to dogs against challenge with virulent canine parvovirus, has been expressed in transgenic plants as an amino‐terminal translational fusion with the GUS gene. Transformants were selected on the basis of their GUS activities, showing expression levels of the recombinant protein up to 3% of the total leaf soluble protein, a production yield comparable to that obtained with the same epitope expressed by chimeric plant viruses. The immunogenicity of the plant‐derived peptide was demonstrated in mice immunized either intraperitoneally or orally with transgenic plant extracts, providing the suitability of the GUS fusions approach for low‐cost production of peptide vaccines.
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