INTERACTION OF NITRIC OXIDE AND TRANSFORMING GROWTH FACTOR-β1 INDUCED BY ANGIOTENSIN II AND MECHANICAL STRETCH IN RAT RENAL TUBULAR EPITHELIAL CELLS
2000; Lippincott Williams & Wilkins; Volume: 164; Issue: 5 Linguagem: Inglês
10.1016/s0022-5347(05)67097-8
ISSN1527-3792
AutoresAkira Miyajima, Jie Chen, Irena Kirman, Dix P. Poppas, E. Darracott Vaughan, Diane Felsen,
Tópico(s)Aldose Reductase and Taurine
ResumoNo AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Nov 2000INTERACTION OF NITRIC OXIDE AND TRANSFORMING GROWTH FACTOR-β1 INDUCED BY ANGIOTENSIN II AND MECHANICAL STRETCH IN RAT RENAL TUBULAR EPITHELIAL CELLS AKIRA MIYAJIMA, JIE CHEN, IRENA KIRMAN, DIX P. POPPAS, E. DARRACOTT VAUGHAN, and DIANE FELSEN AKIRA MIYAJIMAAKIRA MIYAJIMA , JIE CHENJIE CHEN , IRENA KIRMANIRENA KIRMAN , DIX P. POPPASDIX P. POPPAS , E. DARRACOTT VAUGHANE. DARRACOTT VAUGHAN , and DIANE FELSENDIANE FELSEN View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)67097-8AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Changes in intrarenal pressure accompanying unilateral ureteral obstruction can result in tubular mechanical stretch and mediator release from renal tubules. Therefore, we examined the synthesis of nitric oxide and transforming growth factor-β (TGF-β), and their interaction in rat renal epithelial cells (NRK-52E) exposed to either angiotensin II or mechanical stretch. Materials and Methods: NRK-52E were exposed to either angiotensin II or mechanical stretch. Nitrite and TGF-β in the supernatant were assessed by the Greiss reaction and bioassay, respectively. The level of cell hypertrophy and intracellular TGF-β protein was determined by flow cytometry. TGF-β messenger RNA and inducible nitric oxide synthase protein were detected by reverse transcriptase polymerase chain reaction and Western blot, respectively. Results: Angiotensin II stimulated TGF-β1 and nitric oxide. The nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NAME) or angiotensin II type I receptor blocker, losartan, inhibited nitric oxide and TGF-β1 induced by angiotensin II. Flow cytometry showed that either L-NAME or losartan inhibited angiotensin II induced cell hypertrophy. TGF-β1 inhibited iNOS protein and nitric oxide, whereas an anti-TGF-β antibody enhanced iNOS. Mechanical stretch induced TGF-β, inducible NOS protein and nitric oxide. However, TGF-β expression was not affected by L-arginine or L-NAME when cells were exposed to mechanical stretch. Conclusions: These results demonstrate that nitric oxide is an intermediate in angiotensin II stimulated TGF-β1 expression but not in stretch induced TGF-β expression, and that TGF-β1 is a negative regulator of nitric oxide in rat renal epithelial cells. The complex interaction of these cytokines may be a target for intervention in the fibrotic and apoptotic processes in the obstructed kidney. References 1 : Angiotensin II and gene expression in the kidney. Am J Kidney Dis1998; 31: 171. 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Google Scholar From the Department of Urology, Center for Pediatric Urology and Laboratory for Minimally Invasive Urologic Surgery, New York Presbyterian Hospital, Weill Medical College of Cornell University, New York, New York© 2000 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byWang-Rosenke Y, Mika A, Khadzhynov D, Loof T, Neumayer H and Peters H (2018) Stimulation of Soluble Guanylate Cyclase Improves Renal Recovery After Relief of Unilateral Ureteral ObstructionJournal of Urology, VOL. 186, NO. 3, (1142-1149), Online publication date: 1-Sep-2011.Kellner D, Chen J, Richardson I, Seshan S, el Chaar M, Vaughan E, Poppas D and Felsen D (2018) Angiotensin Receptor Blockade Decreases Fibrosis and Fibroblast Expression in a Rat Model of Unilateral Ureteral ObstructionJournal of Urology, VOL. 176, NO. 2, (806-812), Online publication date: 1-Aug-2006.CAVALCANTI A, YUCEL S, DENG D, McANINCH J and BASKIN L (2018) The Distribution of Neuronal and Inducible Nitric Oxide Synthase in Urethral Stricture FormationJournal of Urology, VOL. 171, NO. 5, (1943-1947), Online publication date: 1-May-2004.JOHANSSON R, PANDITA R, POLJAKOVIC M, GARCIA-PASCUAL A, de VENTE J and PERSSON K (2018) Activity and Expression of Nitric Oxide Synthase in the Hypertrophied Rat Bladder and the Effect of Nitric Oxide on Bladder Smooth Muscle GrowthJournal of Urology, VOL. 168, NO. 6, (2689-2694), Online publication date: 1-Dec-2002.MIYAJIMA A, ASANO T, ASANO T, YOSHIMURA I, SETA K and HAYAKAWA M (2018) TRANILAST AMELIORATES RENAL TUBULAR DAMAGE IN UNILATERAL URETERAL OBSTRUCTIONJournal of Urology, VOL. 165, NO. 5, (1714-1718), Online publication date: 1-May-2001.MIYAJIMA A, ITO K, ASANO T, SETA K, UEDA A and HAYAKAWA M (2018) DOES CYCLOOXYGENASE-2 INHIBITOR PREVENT RENAL TISSUE DAMAGE IN UNILATERAL URETERAL OBSTRUCTION?Journal of Urology, VOL. 166, NO. 3, (1124-1129), Online publication date: 1-Sep-2001. Volume 164Issue 5November 2000Page: 1729-1734 Advertisement Copyright & Permissions© 2000 by American Urological Association, Inc.Keywordsnitric oxideureteral obstructionangiotensinstransforming growth factor betaMetricsAuthor Information AKIRA MIYAJIMA More articles by this author JIE CHEN More articles by this author IRENA KIRMAN More articles by this author DIX P. POPPAS More articles by this author E. DARRACOTT VAUGHAN More articles by this author DIANE FELSEN More articles by this author Expand All Advertisement PDF downloadLoading ...
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