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Monoclonal antibody 57B stains tumor tissues that express geneMAGE-A4

2000; Wiley; Volume: 86; Issue: 6 Linguagem: Inglês

10.1002/(sici)1097-0215(20000615)86

1097-0215

Claire Landry, Francis Brasseur, Giulio C. Spagnoli, Étienne Marbaix, Thierry Boon, Pierre G. Coulie, D. Godelaine,

Monoclonal and Polyclonal Antibodies Research

Monoclonal antibody (Mab) 57B, which was raised against a recombinant MAGE-A3 protein, was tested for its ability to stain cells expressing various members of the MAGE-A gene family. COS-7 cells transfected with cDNAs encoding MAGE-A1, A2, A3, A4, A6, or A12 were stained, whereas those transfected with MAGE-A8, A9, A10, or A11 cDNAs were not. However, in tissue sections, we observed a different pattern of staining: the antibody effectively stained the tumors that expressed MAGE-A4 and only these tumors, regardless of the expression of the other MAGE-A genes. It seems, therefore, that at the level of MAGE gene expression found in tumors, a level clearly lower than that observed in transfected COS cells, only the MAGE-A4 protein can be reliably detected. We conclude that the 57B Mab should be useful for tumor diagnosis related to therapeutic vaccination involving MAGE-A4. Int. J. Cancer 86:835–841, 2000. © 2000 Wiley-Liss, Inc.