Evaluation of neuronal cell death by laser scanning cytometry
2002; Elsevier BV; Volume: 9; Issue: 1 Linguagem: Inglês
10.1016/s1385-299x(01)00135-0
ISSN1872-809X
AutoresEster Verdaguer, David Pubill, Víctor Rimbau, Andrés Jiménez, Elena Escubedo, Jordi Camarasa, Mercè Pallàs, Antoni Camins,
Tópico(s)Immune Response and Inflammation
ResumoWe developed a method in which laser scanning cytometry (LSC) is applied to evaluate cell viability. Neuronal cell death induced by glutamic acid, serum potassium deprivation and 3-nitropropionic acid was studied in cerebellar granule cells by neutral red assay (NR) and LSC, using propidium iodide (PI) as fluorescent dye. PI labeled the nuclei of dead neurons and increased fluorescence was measured using a laser scanning cytometer. Similar levels of damage for each injury were detected by NR or LSC. The protocol presented here, provides a fast and sensitive assay for the analysis of neuronal viability using LSC, and can be used to study new neuroprotective drugs in neuronal cell cultures.
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