Methionine transfer RNAs from the blue-green alga Anacystis nidulans
1974; Elsevier BV; Volume: 340; Issue: 2 Linguagem: Inglês
10.1016/0005-2787(74)90105-1
ISSN1879-3002
Autores Tópico(s)Cancer-related gene regulation
ResumoTwo isoaccepting methionine tRNA species of the prokaryote, Anacystis nidulans, can be separated by reversed phase chromatography of the aminoacyl-tRNAs. However, after phenoxyacetylation of the methionyl-tRNAs, another chromatographically distinct species of methionine tRNA can be demonstrated using the same reversed-phase chromatography column. The methionine tRNA species I, accounting for 66% of total acceptor activity can be formylated either by an endogenous Met-tRNA transformylase or by Escherichia coli transformylase. The two other species II and III, accounting for 10 and 24%, respectively, cannot be formylated. Formyl and methionyl acceptance in cross-reactions using heterologous systems from A. nidulans and E. coli were found possible in all combinations. In addition, the algal enzyme preparation was tested for its ability to aminoacylate and formylate tRNAMeti from wheat germ. This cytoplasmic initiator tRNA was partially recognized by the algal transformylase, whereas the E. coli transformylase showed no recognition of this species (see also Leis, J.P. and Keller, E.B. (1970) Proc. Natl.Acad. Sci. U.S. 67, 1593–1599).
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