Selective modulation of specific protein kinase C (PKC) isoforms in primary human megakaryocytic vs. erythroid cells
1999; Wiley; Volume: 255; Issue: 1 Linguagem: Inglês
10.1002/(sici)1097-0185(19990501)255
ISSN1097-0185
AutoresMarco Marchisio, Valeria Bertagnolo, Claudio Celeghini, Marco Vitale, Silvano Capitani, Giorgio Zauli,
Tópico(s)Chronic Lymphocytic Leukemia Research
ResumoWe have investigated the pattern of expression of classical (alpha, betaI, betaII, gamma), novel (delta) and atypical (zeta) protein kinase C (PKC) isoforms during the course of human hematopoietic differentiation along the closely related megakaryocytic and erythroid lineages. Using in situ immunofluorescence analysis, freshly isolated human pluripotent CD34+ hematopoietic progenitor cells expressed detectable amounts of all the PKC isoforms investigated. On the other hand, clear-cut differences in terms of PKC staining were noticed between cells belonging to the erythroid and megakaryocytic lineages, obtained after 9 days of serum-free liquid culture in the presence of specific growth factors. Specifically, 1) erythroid cells showed a very weak expression of PKC-alpha, -betaI, -betaII, and -gamma, while megakaryocytes showed an enhanced expression of all classical PKC isoforms, predominantly confined to the cytoplasm; 2) the expression of PKC-delta increased in the cytoplasmic and nuclear compartments of both erythroid and megakaryocytic cells with respect to CD34+ cells; and 3) atypical PKC-zeta isoform showed a striking accumulation in the nucleus during both erythroid and megakaryocytic differentiation.
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