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Analysis of antibiotics in urine and wipe samples from environmental and biological monitoring—Comparison of HPLC with UV-, single MS- and tandem MS-detection

2005; Elsevier BV; Volume: 831; Issue: 1-2 Linguagem: Inglês

10.1016/j.jchromb.2005.11.030

1873-376X

Jochen Tuerk, Marius Reinders, Dennis Dreyer, Thekla Kiffmeyer, K. G. Schmidt, Heinz‐Martin Kuß,

Biosensors and Analytical Detection

Results of the simultaneous determination of the structurally different antibiotics cefazoline, cefotiame, cefuroxime, chloramphenicol, ciprofloxacin, ofloxacin, sulfamethoxazole and trimethoprim from environmental and biological monitoring using high-performance liquid chromatography with UV, single mass and tandem mass spectrometry were compared. For sample enrichment and clean-up a SPE method using bakerbond C18 cartridges was developed. Mean recovery rates were above 70%. Because of the complex urine matrix, only the wipe samples could be analyzed by UV-detection. However, UV-detection and single MS-detection are useful for control measurements after spillage, e.g. (LOD = 1–2 ng/cm2). Samples from biological monitoring of occupational uptake should be analyzed by LC–MS/MS. The limits of detection (LOD) in urine ranged from 0.4 to 70 μg/L for LC–MS and 0.01 to 0.9 μg/L for LC–MS/MS detection. The limits of detection in wipe samples ranged from 0.003 to 0.13 ng/cm2.