Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacterium Geobacter sulfurreducens
2013; Wiley; Volume: 587; Issue: 16 Linguagem: Inglês
10.1016/j.febslet.2013.07.003
ISSN1873-3468
AutoresJoana M. Dantas, Diogo M. Tomaz, Leonor Morgado, Carlos A. Salgueiro,
Tópico(s)Electrochemical Analysis and Applications
ResumoThe cytochrome PccH from Geobacter sulfurreducens ( Gs ) plays a crucial role in current‐consuming fumarate‐reducing biofilms. Deletion of pccH gene inhibited completely electron transfer from electrodes toward Gs cells. The pccH gene was cloned and the protein heterologously expressed in Escherichia coli . Complementary biophysical techniques including CD, UV–visible and NMR spectroscopy were used to characterize PccH. This cytochrome contains one low‐spin c ‐type heme with His–Met axial coordination and unusual low‐reduction potential. This reduction potential is pH‐dependent, within the Gs physiological pH range, and is discussed within the context of the electron transfer mechanisms from electrodes to Gs cells.
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