Artigo Revisado por pares

APHERESIS PLATELET CONCENTRATES: CORRELATION OF DAY ONE LEVELS OF IN VITRO QUALITY MARKERS WITH CORRESPONDING LEVELS ON DAYS TWO TO FIVE OF STORAGE

1996; Elsevier BV; Volume: 84; Issue: 5 Linguagem: Inglês

10.1016/s0049-3848(96)00201-0

ISSN

1879-2472

Autores

Hagop Bessos, Anne Atkinson, Adam McGill, J. Kenneth Baillie, Jerhard Seghatchian, Maggie Vickers, Debbie Bishop, N. P. Tandy, William G. Murphy,

Tópico(s)

Blood groups and transfusion

Resumo

Platelet concentrates (PCs) for transfusion can be produced in three ways: conventional platelet rich plasma technique; pooled buffy coat method; or using apheresis technology [ 1 KICKLER T.S. Improving the quality of stored platelets. Transfusion. 1991; 31: 1-3 Crossref PubMed Scopus (12) Google Scholar , 2 ERSON N.A.B. PAMPHILON D.H. TANDT N.J. SAUNDERS J. FRASER I.D. Comparison of platelet-rich plasma collection using the haemonatics PCS and Baxter autopheresis C. Vox Sang. 1991; 60: 155-158 Crossref PubMed Scopus (11) Google Scholar , 3 DZIK W.H. Current Trends: Preparation of platelet concentrates. Transfus. Sci. 1991; 12: 171-181 Abstract Full Text PDF PubMed Scopus (2) Google Scholar , 4 BERTOLINI F. REBULLA P. PORRETTI L. MURPHY S. Platelet quality after 15-day storage of platelet concentrates prepared from buffy coats and stored in a glucose-free crystalloid medium. Transfusion. 1992; 32: 9-16 Crossref PubMed Scopus (56) Google Scholar ]. The use of apheresis PC obtained from single donors is becoming increasingly popular principally due to decreased exposure of recipients to infectious agents, WBC antigens, and donor platelet antigens [ 5 BOCK M. GAWAZ M.P. DIETZLER A. HEIM M.U. MEMPEL W. Single-donor platelet concentrates: Changes of surface glycoproteins during storage. Haemostasis. 1994; 24: 230-235 PubMed Google Scholar ]. Platelet concentrates are stored at room temperature until transfused or discarded at the end of their shelf life (currently five days). During storage the PCs undergo changes that result in increased or decreased levels of various in vitro markers, which is usually associated with diminution of quality [ 6 HOLME S. MOROFF G. WHITELY P. HALLINEN D. HEATON A. A. Properties of platelet concentrates prepared after extended whole blood holding time. Transfusion. 1989; 29: 689-692 Crossref PubMed Scopus (30) Google Scholar , 7 CESAR J.M. GARCIA-AVELLO A. MONTEAGUDO J. ESPINOSA J.I. LODOS J.C. CASTILLO R. NAVARRO J.L. Von Willebrand factor availability in platelet concentrates stored for 5 days. Am. J. Haematol. 1994; 45: 109-111 Crossref PubMed Scopus (8) Google Scholar ]. One recently employed marker is glycocalicin (GC), a major segment of glycoprotein Ib, which is released during the storage of PC [ 8 BESSOS H. MURPHY W.G. A new competitive binding enzyme-linked immunosorbent assay for glycocalicin in plasma and platelet concentrate supernatants. Thromb. Res. 1990; 59: 497-507 Abstract Full Text PDF PubMed Scopus (18) Google Scholar , 9 BESSOS H. MURPHY W.G. ROBERTSON A. VICKERS M. SEGHATCHIAN M.J. TANDY N.P. CUTTS M. PAMPHILON D.H. Quality of platelet concentrates irradiated with UVB light: Effect of UV dose and dose rate on glycocalicin release and correlation with other markers of the platelet storage lesion. Transfusion Med. 1993; 3: 115-121 Crossref PubMed Scopus (14) Google Scholar ]. The released GC levels in PC supernatants correlate significantly with traditional markers of PC quality [ 9 BESSOS H. MURPHY W.G. ROBERTSON A. VICKERS M. SEGHATCHIAN M.J. TANDY N.P. CUTTS M. PAMPHILON D.H. Quality of platelet concentrates irradiated with UVB light: Effect of UV dose and dose rate on glycocalicin release and correlation with other markers of the platelet storage lesion. Transfusion Med. 1993; 3: 115-121 Crossref PubMed Scopus (14) Google Scholar ]. In this study we monitored the levels of GC and other in vitro markers during the storage of apheresis PC and determined the correlation of day one marker levels with corresponding levels on days two to five. The purpose of this correlation was to assess the predictive value of day 1 marker levels towards the quality of apheresis PC throughout the storage period.

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