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A Polypodium leucotomos extract inhibits solar‐simulated radiation‐induced TNF‐α and iNOS expression, transcriptional activation and apoptosis

2007; Wiley; Volume: 16; Issue: 10 Linguagem: Inglês

10.1111/j.1600-0625.2007.00603.x

1600-0625

Agnieska Jańczyk, María Ángeles García-López, Pablo Fernández‐Peñas, José L. Alonso-Lebrero, Ignacio Benedicto, Manuel López‐Cabrera, Salvador González,

Essential Oils and Antimicrobial Activity

Abstract: In this report, we have examined the molecular basis of the photoprotective effect of a hydrophilic extract of the fern Polypodium leucotomos (PL) in vitro , using a solar simulator as the source of UV radiation (SSR). We found that pretreatment of human keratinocytes with PL inhibited SSR‐mediated increase of tumor necrosis factor (TNF)‐α and also abrogated nitric oxide (NO) production. Consistent with this, PL blocked the induction of inducible nitric oxide synthase (iNOS) elicited by SSR. In addition, PL inhibited the SSR‐mediated transcriptional activation of NF‐κB and AP1. Finally, we demonstrated that pretreatment with PL exerted a cytoprotective effect against SSR‐induced damage, resulting in increased cell survival. Together, these data postulate a multifactor mechanism of protection not exclusively reliant on the antioxidant capability of PL, and strengthen the basic knowledge on the photoprotective effect of this botanical agent.