Clofarabine induces hypomethylation of DNA and expression of Cancer-Testis antigens
2009; Elsevier BV; Volume: 33; Issue: 12 Linguagem: Inglês
10.1016/j.leukres.2009.04.005
ISSN1873-5835
AutoresYana Zhang, Masum Shahriar, Jian Zhang, Sharif Ahmed, Seah H. Lim,
Tópico(s)Genomics and Chromatin Dynamics
ResumoIn this study, treatment of lymphoid tumor cells with low dose clofarabine upregulated the expression of Sp17 and SPAN-Xb. This was associated with an increase in hypomethylated CpG dinucleotides and a decrease in global DNA methylation, as demonstrated by decreases in the percent of methylated Alu repeats. The most optimal concentration of clofarabine to induce DNA hypomethylation and CT antigen expression was between 1x10(-9) and 1x10(-8)M. Above this, clofarabine resulted in tumor cell growth inhibition and apoptosis. Our results provide the first evidence for the CT antigen-inducing and DNA hypomethylating property of low concentration clofarabine.
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