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Halorientalis persicus sp. nov., an extremely halophilic archaeon isolated from a salt lake and emended description of the genus Halorientalis

2013; Microbiology Society; Volume: 64; Issue: Pt_3 Linguagem: Inglês

10.1099/ijs.0.058164-0

1466-5034

Mohammad Ali Amoozegar, Ali Makhdoumi, Maliheh Mehrshad, Seyed Abolhassan Shahzadeh Fazeli, Cathrin Spröer, António Ventosa,

Bacterial Identification and Susceptibility Testing

An extremely halophilic archaeon, strain D108 T , was isolated from a brine sample of Aran-Bidgol salt lake in Iran. The novel strain was cream-pigmented, motile, pleomorphic rod-shaped and required at least 2.5 M NaCl but not MgCl 2 for growth. Optimal growth was achieved with 4.3 M NaCl and 0.1 M MgCl 2 . The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, and the strain was able to grow over a pH range of 6.5 to 9.0, and a temperature range of 30 to 50 °C. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain D108 T clustered with the type strain of the sole species of the genus Halorientalis , Halorientalis regularis TNN28 T , with a sequence similarity of 98.8 %. The polar lipid pattern of strain D108 T consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, one phosphoglycolipid and two glycolipids. The only quinone present was MK-8(II-H 2 ). The DNA G + C content of strain D108 T was 62.8 mol%. DNA–DNA hybridization studies (45 % with Halorientalis regularis IBRC-M 10760 T ), as well as biochemical and physiological characterization, allowed strain D108 T to be differentiated from Halorientalis regularis . A novel species of the genus Halorientalis , Halorientalis persicus sp. nov., is therefore proposed to accommodate this strain. The type strain is D108 T ( = IBRC-M 10043 T = CECT 8375 T ). An emended description of the genus Halorientalis is also proposed.