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Analysis of cis- and trans-acting elements in the hormone-sensitive human somatotropin gene promoter

1989; Pergamon Press; Volume: 34; Issue: 1-6 Linguagem: Inglês

10.1016/0022-4731(89)90068-x

1878-2353

Frédéric P. Lemaigre, Stéphane Courtois, Serge Durviaux, Christine J. Egan, Dominique A. Lafontaine, Guy G. Rousseau,

Adipose Tissue and Metabolism

The expression of the human growth hormone (hGH) gene is regulated by several transcription factors. Basal level transcription factors include TATA box-binding proteins, Sp1, USF and CTF/NF-1. The hGH gene is expressed only in pituitary somatotrophs, and the pituitary-specific GHF-1/Pit-1 protein is a potent transcriptional stimulator. Glucocorticoid and thyroid hormones, insulin, and GHRH which acts via cAMP, also control hGH gene transcription via trans-acting factors some of which are the hormone receptors themselves. Three transcription initiation sites were detected when hGH gene promoter activity was studied in a cell-free system. This system enabled us to delineate the respective role of some transcription factors and to propose a model that accounts for the basal, pituitary-specific, and hormonal control of hGH gene expression.