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Bone morphogenetic protein-2 promotes dissociated effects on the number and differentiation of cultured ventral mesencephalic dopaminergic neurons

1999; Wiley; Volume: 38; Issue: 2 Linguagem: Inglês

10.1002/(sici)1097-4695(19990205)38

1097-4695

J. Reiriz, Mònica Espejo, Francesc Ventura, Santiago Ambrosio, Jordi Alberch,

Heterotopic Ossification and Related Conditions

Journal of NeurobiologyVolume 38, Issue 2 p. 161-170 Fast Track Bone morphogenetic protein-2 promotes dissociated effects on the number and differentiation of cultured ventral mesencephalic dopaminergic neurons Julia Reiriz, Julia Reiriz Departament Infermeria Fonamental i Mèdic-Quirúrgic, Escola Universitària d'Infermeria, Universitat de Barcelona, Feixa Llarga s/n, 08907, L'Hospitalet de Llobregat, Barcelona, Spain Departament Biologia Cel.lular i Anatomia Patològica, Facultat de Medicina, IDIBAPS, Universitat de Barcelona, Casanova 143, 08036 Barcelona, SpainSearch for more papers by this authorMonica Espejo, Monica Espejo Departament Ciències Fisiològiques, Facultat d'Odontologia, Universitat de Barcelona, Feixa Llarga s/n, 08907, L'Hospitalet de Llobregat, Barcelona, SpainSearch for more papers by this authorFrancesc Ventura, Francesc Ventura Departament Ciències Fisiològiques, Facultat d'Odontologia, Universitat de Barcelona, Feixa Llarga s/n, 08907, L'Hospitalet de Llobregat, Barcelona, SpainSearch for more papers by this authorSantiago Ambrosio, Santiago Ambrosio Departament Ciències Fisiològiques, Facultat d'Odontologia, Universitat de Barcelona, Feixa Llarga s/n, 08907, L'Hospitalet de Llobregat, Barcelona, SpainSearch for more papers by this authorJordi Alberch, Corresponding Author Jordi Alberch Departament Biologia Cel.lular i Anatomia Patològica, Facultat de Medicina, IDIBAPS, Universitat de Barcelona, Casanova 143, 08036 Barcelona, SpainDepartament Biologia Cel.lular i Anatomia Patològica, Facultat de Medicina, IDIBAPS, Universitat de Barcelona, Casanova 143, 08036 Barcelona, SpainSearch for more papers by this author Julia Reiriz, Julia Reiriz Departament Infermeria Fonamental i Mèdic-Quirúrgic, Escola Universitària d'Infermeria, Universitat de Barcelona, Feixa Llarga s/n, 08907, L'Hospitalet de Llobregat, Barcelona, Spain Departament Biologia Cel.lular i Anatomia Patològica, Facultat de Medicina, IDIBAPS, Universitat de Barcelona, Casanova 143, 08036 Barcelona, SpainSearch for more papers by this authorMonica Espejo, Monica Espejo Departament Ciències Fisiològiques, Facultat d'Odontologia, Universitat de Barcelona, Feixa Llarga s/n, 08907, L'Hospitalet de Llobregat, Barcelona, SpainSearch for more papers by this authorFrancesc Ventura, Francesc Ventura Departament Ciències Fisiològiques, Facultat d'Odontologia, Universitat de Barcelona, Feixa Llarga s/n, 08907, L'Hospitalet de Llobregat, Barcelona, SpainSearch for more papers by this authorSantiago Ambrosio, Santiago Ambrosio Departament Ciències Fisiològiques, Facultat d'Odontologia, Universitat de Barcelona, Feixa Llarga s/n, 08907, L'Hospitalet de Llobregat, Barcelona, SpainSearch for more papers by this authorJordi Alberch, Corresponding Author Jordi Alberch Departament Biologia Cel.lular i Anatomia Patològica, Facultat de Medicina, IDIBAPS, Universitat de Barcelona, Casanova 143, 08036 Barcelona, SpainDepartament Biologia Cel.lular i Anatomia Patològica, Facultat de Medicina, IDIBAPS, Universitat de Barcelona, Casanova 143, 08036 Barcelona, SpainSearch for more papers by this author First published: 28 January 1999 https://doi.org/10.1002/(SICI)1097-4695(19990205)38:2 3.0.CO;2-3Citations: 45AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Bone morphogenetic proteins (BMPs) are a family of growth differentiation factors which induce bone formation from mesenchymal cells. These proteins are members of the transforming growth factor-β superfamily. The expression of BMPs in the nervous system as well as in other tissues has been reported. In this study, we show that the presence of BMP-2 resulted in a dose-dependent increase in the number of tyrosine hydroxylase-immunoreactive ventral mesencephalic cells after 7 days in serum-free medium cultures. A maximal response was elicited at 10 ng/mL. BMP-2 also increased the number of primary neurites and branch points as well as the length of the longest neurite in a dose-dependent manner, with a maximal effect at 1 ng/mL. In contrast, BMP-2 did not modify the number or the function of GABAergic neurons. On the other hand, we observed stimulation of proliferation and morphological changes in glial cells (astrocytes become more fibrous shaped) in the presence of a high BMP-2 concentration (100 ng/mL), but not with lower doses, suggesting that the neurotrophic effect in dopaminergic neurons is not mediated by astroglial cells. This is consistent with the fact that the BMP-2 effect on dopaminergic neurons was observed even when the cultures were treated with α-aminoadipic acid to exclude the presence of glial cells. In summary, our data indicate that BMP-2 is a potent neurotrophic factor for ventral mesencephalic dopaminergic cells in culture. © 1999 John Wiley & Sons, Inc. J Neurobiol 38: 161–170, 1999 Citing Literature Volume38, Issue25 February 1999Pages 161-170 RelatedInformation