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BIBTEX RIS

The phage K lytic enzyme LysK and lysostaphin act synergistically to kill MRSA

2008; Oxford University Press; Volume: 287; Issue: 2 Linguagem: Inglês

10.1111/j.1574-6968.2008.01308.x

ISSN

1574-6968

Autores

Stephen C. Becker, Juli Foster‐Frey, David M. Donovan,

Tópico(s)

Bacterial Genetics and Biotechnology

Resumo

LysK is the endolysin from the staphylococcal bacteriophage K, and can digest the cell wall of many staphylococci. Lysostaphin is a bacteriocin secreted by Staphylococcus simulans to kill Staphylococcus aureus. Both LysK and lysostaphin have been shown to lyse methicillin-resistant S. aureus (MRSA). This study describes optimal reaction conditions for the recombinant His-tagged LysK protein (pH range pH 6-10, and 0.3-0.5 M NaCl), and C-His-LysK MIC (32.85+/-4.87 mug mL(-1)). LysK and lysostaphin demonstrate antimicrobial synergy by the checkerboard assay.

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