Artigo Revisado por pares

Acyl coenzyme a inhibition of Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase: A comparison of the TPN and DPN linked reactions

1973; Elsevier BV; Volume: 53; Issue: 1 Linguagem: Inglês

10.1016/0006-291x(73)91401-0

ISSN

1090-2104

Autores

Elmon L. Coe, Lung-Hsiung Hsu,

Tópico(s)

Pneumocystis jirovecii pneumonia detection and treatment

Resumo

The inhibitory effects of ATP, coenzyme A, and acetyl, malonyl, and oleyl derivatives of coenzyme A on the TPN and DPN dependent activities of Leuconostoc glucose-6-phosphate dehydrogenase are compared. At pH 7.8, 24°, saturating levels of DPN or TPN, and inhibitor concentrations of 2–4 mM only ATP has an appreciable effect on the TPN dependent reaction, but all were potent inhibitors of the DPN dependent reaction. Oleyl coenzyme A was the most effective (Ki ∼ 0.15 mM against glucose-6-phosphate) while acetyl coenzyme A was least effective (Ki ∼ 1.0 mM). A possible regulatory role of this inhibition in fatty acid synthesis is suggested.

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