Long-Term Culture of Fetal Liver Cells Using a Three-Dimensional Porous Polymer Substrate
2000; Lippincott Williams & Wilkins; Volume: 46; Issue: 4 Linguagem: Inglês
10.1097/00002480-200007000-00005
ISSN1538-943X
AutoresHirotoshi Miyoshi, Tomo Ehashi, Hideo Ema, Hsiang Chun Hsu, Hiromitsu Nakauchi, Norio Ohshima,
Tópico(s)Clinical Nutrition and Gastroenterology
ResumoTo develop a bioartificial liver, long-term culture of fetal liver cells over a month’s time was performed under three different culture conditions, i.e., stationary cultures and shaken-flask cultures, both by using a substratum made of porous polyvinyl formal (PVF) resin and conventional monolayer dish cultures as controls. Time course changes in cell numbers and albumin secretion were evaluated in cultures using Williams’ E medium (WE) or minimum essential medium alpha (aMEM) supplemented with serum and hormones. In the WE medium, the numbers of fetal liver cells in all culture conditions gradually decreased with time, and albumin secretion rates rapidly decreased. In the stationary cultures using PVF, however, a significant increase in albumin secretion was observed after two weeks of culture. When cells were cultured in aMEM, the fetal liver cells exhibited sufficient proliferation in stationary and monolayer cultures, although albumin secretion rates per single cell were lower than those in WE. On the basis of these results, another series of culture experiments were performed, in which aMEM was used for the first 10 days to encourage cell proliferation, and the medium was changed to WE afterward. In these cultures, albumin secretion rates in the stationary cultures dramatically increased after the medium exchanges and were maintained at these high levels throughout the remaining culture period.
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