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The identification of vesicular-arbuscular mycorrhizal fungi using immunofluorescence

1983; Elsevier BV; Volume: 15; Issue: 4 Linguagem: Inglês

10.1016/0038-0717(83)90009-3

1879-3428

Jill M. Wilson, MJ Trinick, C.A. Parker,

Plant Pathogens and Fungal Diseases

An antiserum was produced to a species of Gigaspora using cell wall antigen from hyphae collected from germinating spores. Using this antiserum in the fluorescent antibody (FA) technique, vesicular-arbuscular mycorrhizal (VAM) fungi could easily be distinguished from non-endogonaceous fungi. Differentiation among different VAM fungi, though not clear cut, was possible, and specificity was improved by absorption of the serum with hyphae of Acaulospora laevis. Variations in staining reactions between different fungi suggest that wall structure interferes with the expression of the FA test.