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Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro

2014; Multidisciplinary Digital Publishing Institute; Volume: 7; Issue: 2 Linguagem: Inglês

10.3390/ma7021342

1996-1944

Achim Salamon, Sandra Van Vlierberghe, Ine Van Nieuwenhove, Frank Baudisch, Geert‐Jan Graulus, Verena Benecke, Kristin Alberti, Hans‐Georg Neumann, Joachim Rychly, José C. Martins, Peter Dubruel, Kirsten Peters,

Mesenchymal stem cell research

Due to the weak regeneration potential of cartilage, there is a high clinical incidence of articular joint disease, leading to a strong demand for cartilaginous tissue surrogates. The aim of this study was to evaluate a gelatin-based hydrogel for its suitability to support chondrogenic differentiation of human mesenchymal stem cells. Gelatin-based hydrogels are biodegradable, show high biocompatibility, and offer possibilities to introduce functional groups and/or ligands. In order to prove their chondrogenesis-supporting potential, a hydrogel film was developed and compared with standard cell culture polystyrene regarding the differentiation behavior of human mesenchymal stem cells. Cellular basis for this study were human adipose tissue-derived mesenchymal stem cells, which exhibit differentiation potential along the adipogenic, osteogenic and chondrogenic lineage. The results obtained show a promotive effect of gelatin-based hydrogels on chondrogenic differentiation of mesenchymal stem cells in vitro and therefore encourage subsequent in vivo studies.