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Recent advances in proteolysis and peptide/protein separation by chromatographic strategies

2010; Springer Science+Business Media; Volume: 53; Issue: 4 Linguagem: Inglês

10.1007/s11426-010-0135-7

1674-7291

Xiangmin Zhang, Baohong Liu, Lihua Zhang, Hanfa Zou, Jing Cao, Mingxia Gao, Jia Tang, Yun Liu, Pengyuan Yang, Yukui Zhang,

Glycosylation and Glycoproteins Research

This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chromatographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.